Difference between revisions of "Part:BBa K1997015"

 
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<partinfo>BBa_K1997015 short</partinfo>
 
<partinfo>BBa_K1997015 short</partinfo>
  
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This part is a positive control of GFP with the complete ZFN.
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This part is an integrated tool for protein-protein interaction research using split-GFP system as reporter. the "Zif268" subpart can be easily replaced using Golden Gate technique with BsaI
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:01, 14 October 2016


P+R->sGFP-N->Zif268->sGFP-C->TER

This part is an integrated tool for protein-protein interaction research using split-GFP system as reporter. the "Zif268" subpart can be easily replaced using Golden Gate technique with BsaI


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 832
    Illegal AgeI site found at 916
    Illegal AgeI site found at 1000
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1003
    Illegal BsaI.rc site found at 736
    Illegal BsaI.rc site found at 1217