Difference between revisions of "Part:BBa I15010"
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| − | Requires PCB biosynthetic genes ( | + | Requires PCB biosynthetic genes (BBa_I15008 and BBa_I15009) for chromophore formation. The light responsive domain (Cph1) has maximal response to light near 660nm. Exposure to red light inhibits the activity of the EnvZ histidine kinase domain. When active (in the dark) EnvZ phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpC promoter and represses transcription from the OmpF promoter. Must be used in E.coli deficient in natural EnvZ. |
Some user-experiences and characterization experiments related to the bacterial photography system are documented here [http://openwetware.org/wiki/Talk:BE.109:Systems_engineering/RT-PCR_data_analysis]. In general, experiments were conducted by students in BE.109, spring 2006, and involved variation of the strain's growth conditions or light exposure. The consequences of each experimental variation were followed with b-gal assay and RT-PCR. | Some user-experiences and characterization experiments related to the bacterial photography system are documented here [http://openwetware.org/wiki/Talk:BE.109:Systems_engineering/RT-PCR_data_analysis]. In general, experiments were conducted by students in BE.109, spring 2006, and involved variation of the strain's growth conditions or light exposure. The consequences of each experimental variation were followed with b-gal assay and RT-PCR. | ||
Revision as of 13:20, 2 July 2007
cph8 (Cph1/EnvZ fusion)
Chimeric Cph1 light receptor/EnvZ protein.
Usage and Biology
Requires PCB biosynthetic genes (BBa_I15008 and BBa_I15009) for chromophore formation. The light responsive domain (Cph1) has maximal response to light near 660nm. Exposure to red light inhibits the activity of the EnvZ histidine kinase domain. When active (in the dark) EnvZ phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpC promoter and represses transcription from the OmpF promoter. Must be used in E.coli deficient in natural EnvZ.
Some user-experiences and characterization experiments related to the bacterial photography system are documented here [http://openwetware.org/wiki/Talk:BE.109:Systems_engineering/RT-PCR_data_analysis]. In general, experiments were conducted by students in BE.109, spring 2006, and involved variation of the strain's growth conditions or light exposure. The consequences of each experimental variation were followed with b-gal assay and RT-PCR.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2257
Illegal XhoI site found at 364 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]