Difference between revisions of "Part:BBa K2020042:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | tRNA has 77 amino acids. The ncAA will be attached to the 3' end. On the contrary to aminoacylation with natural amino acids, the acceptor minihelix alone is a substrate for aminoacylation with ncAA's. | |
===Source=== | ===Source=== | ||
Revision as of 22:51, 13 October 2016
Mj-tRNA with amber anticodon for incorporating ncAA in E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
tRNA has 77 amino acids. The ncAA will be attached to the 3' end. On the contrary to aminoacylation with natural amino acids, the acceptor minihelix alone is a substrate for aminoacylation with ncAA's.
Source
wild type tyrosyl Methanococcus janaschii tRNA:Synthetase-pair with anticodon changed to Amber Anticodon CTA. Obtained via iGEM Team 2014 Austin, Texas.
References
- Zhang et al, 2002, Structure-based design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase for incorporation of O-methyl-L-tyrosine
- Steer et al, 1999, Major Anticodon-binding Region Missing from an Archaebacterial tRNA Synthetase