Difference between revisions of "Part:BBa K2116001:Design"
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===Design Notes=== | ===Design Notes=== | ||
The initial addgene sequence has an internal stop codon and thus noncoding regions, which were removed during construction. | The initial addgene sequence has an internal stop codon and thus noncoding regions, which were removed during construction. | ||
| + | Has a T-->Y mutation at position 31, which doesn't effect it's function as far as we could determine. | ||
Latest revision as of 22:05, 13 October 2016
Transcriptional regulator esaR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The initial addgene sequence has an internal stop codon and thus noncoding regions, which were removed during construction. Has a T-->Y mutation at position 31, which doesn't effect it's function as far as we could determine.
Source
The original source is the plant pathogen []. We have obtained the D91G variant from Cynthia Collins through Addgene (Addgene plasmid # 47646).