Difference between revisions of "Part:BBa K1968008"

Revision as of 21:33, 13 October 2016

A widely used constitutive promoter, ErmE (promoter region of erythromycin-resistance gene) was isolated from Streptomycin (Bibb et al., 1985). It works with related gram positive actinobacteria, such as Rhodococcus. We have this part in pUPD2, a MoClo-compatible level 0 vector with no illegal sites for MoClo assembly standard, GoldenBraid assembly standard and iGEM’s pairwise assembly standard.

References

Bibb, M.J., Janssen, G.R. and Ward, J.M., 1985. Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus. Gene, 38, pp.215-226.

Tong, Y., Charusanti, P., Zhang, L., Weber, T. and Lee, S.Y., 2015. CRISPR-Cas9 based engineering of actinomycetal genomes. ACS synthetic biology, 4, pp.1020-1029.

ErmE promoter Phytobrick

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 A widely used constitutive promoter, ErmE (promoter region of erythromycin-resistance gene) was isolated from Streptomycin (Bibb et al., 1985).  It works with related gram positive actinobacteria, such as Rhodococcus.  We have this part in pUPD2, a MoClo-compatible level 0 vector with no illegal sites for MoClo assembly standard, GoldenBraid assembly standard and iGEM’s pairwise assembly standard.
+===References===
+Bibb, M.J., Janssen, G.R. and Ward, J.M., 1985. Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus. Gene, 38, pp.215-226.
+Tong, Y., Charusanti, P., Zhang, L., Weber, T. and Lee, S.Y., 2015. CRISPR-Cas9 based engineering of actinomycetal genomes. ACS synthetic biology, 4, pp.1020-1029.