Difference between revisions of "Part:BBa K2020042:Experience"

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Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a low copy plasmid (e.g. pACYC) which fits your needs.
 
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[http://2016.igem.org/Team:Aachen Team Aachen 2016] used tRNA in pACYC with p15A-ORI, Ipp-Promoter, Terminator, Gent-Resistance. Together with an ncAA-Synthetase (For example: BBa_K2020050) with own promoter and Terminator.
 

Latest revision as of 21:26, 13 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2020042

[http://2014.igem.org/Team:Austin_Texas/kit iGEM Team Austin, Texas 2016 - Expanded Genetic Code Measurement Kit]

The tRNA is used together with a tRNA-Synthetase. It has been proven to work with (enter links for parts)

  • Y-RS
  • oNBY-RS
  • AzF
  • CN-F synthetase
  • Iodo-Y synthetase
  • 5HT-P synthetase
  • Nitro-Y synthetase
  • Amino-Y synthetase

[http://2014.igem.org/Team:Austin_Texas/photocage iGEM Team Austin,, Texas - Project: Photocaged T7 RNA Polymerase]

iGEM Team Austin, Texas 2014 insorporated oNBY into T7 RNA polymerase.

[http://2016.igem.org/Team:Aachen/Description iGEM-Team Aachen 2016 - LIPS Light inducible proteases]

iGEM Team Aachen 2016 used the tRNA to successfully incorporate tyrosine, oNB-Y and in E.coli BL21 DE3 gold. Furthermore they used the tRNA with the pFRY reporter for screening their newly designed DMNBS-RS.

User Reviews

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