Difference between revisions of "Part:BBa K2148017"

 
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This part contains the 3'UTR for the rbcL gene found in Chlamydomonas reinhardtii chloroplast genome and has the homology regions right from the trnE2-psbH integenic region with psaA promoter. This is coded as a level-0 3UTR/TERM Phytobrick, which together with other level-0 Phytobrick can form a desired transcriptional unit for transformation, which would also be available for Homologous Recombination.
 
This part contains the 3'UTR for the rbcL gene found in Chlamydomonas reinhardtii chloroplast genome and has the homology regions right from the trnE2-psbH integenic region with psaA promoter. This is coded as a level-0 3UTR/TERM Phytobrick, which together with other level-0 Phytobrick can form a desired transcriptional unit for transformation, which would also be available for Homologous Recombination.
  
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===Usage and Biology===
 
===Usage and Biology===
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This is a level-0 3UTR/TERM Phytobrick conforming to the Phytobrick Standard. Along with other level-0 Phytobrick, it can be used to create a desired transcriptional unit to transform Chlamydomonas reinhardtii choloroplast.
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<partinfo>BBa_K2148017 parameters</partinfo>
 
<partinfo>BBa_K2148017 parameters</partinfo>
 
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===Characterisation===
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===References===
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Wannathong T, Waterhouse JC, Young REB, Economou CK, Purton S. New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology. 2016;100:5467-5477. doi:10.1007/s00253-016-7354-6.

Revision as of 19:51, 13 October 2016


C.reinhardtii chloroplast rbcL 3UTR w/ Homologies

This part contains the 3'UTR for the rbcL gene found in Chlamydomonas reinhardtii chloroplast genome and has the homology regions right from the trnE2-psbH integenic region with psaA promoter. This is coded as a level-0 3UTR/TERM Phytobrick, which together with other level-0 Phytobrick can form a desired transcriptional unit for transformation, which would also be available for Homologous Recombination.


Usage and Biology

This is a level-0 3UTR/TERM Phytobrick conforming to the Phytobrick Standard. Along with other level-0 Phytobrick, it can be used to create a desired transcriptional unit to transform Chlamydomonas reinhardtii choloroplast.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 515
    Illegal NheI site found at 1944
    Illegal NheI site found at 2265
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 77
    Illegal BsaI.rc site found at 2670



Characterisation

References

Wannathong T, Waterhouse JC, Young REB, Economou CK, Purton S. New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology. 2016;100:5467-5477. doi:10.1007/s00253-016-7354-6.