Difference between revisions of "Part:BBa K2148015:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The target sequence was generated by Alex Mayorov using Benchling software and the scaffold sequence was obtained from Addgene.
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The target sequence was generated by Alex Mayorov using Benchling software and the scaffold sequence was obtained from Addgene. Two additional Guanine after the transcription start site is added to provide stability for the target RNA on advice from Dr Nikola Patron (Earlham Institute).
  
  

Latest revision as of 19:28, 13 October 2016


gRNA for AGG PAM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The target sequence was generated by Alex Mayorov using Benchling software and the scaffold sequence was obtained from Addgene. Two additional Guanine after the transcription start site is added to provide stability for the target RNA on advice from Dr Nikola Patron (Earlham Institute).


Source

Chlamydomonas reinhardtii

References