Difference between revisions of "Part:BBa K2120311"

Revision as of 18:50, 13 October 2016

B0015+tetR+B0034+pBAD-AraC+B0015+ptet+B0034+RFP+B0015 pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And tetR is an inhibitor which is built to repress the expression of ptet promoter. We use it to control the “on” or ”off” state of toxin gene. RFP is the reporter gene. In order to test these do not interfere with each other , We reserve 【BBa_K2120303】 .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 911
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1076
Illegal AgeI site found at 2839
Illegal AgeI site found at 2951
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1093

Revision as of 18:49, 13 October 2016 (view source) YangHongYu (Talk \| contribs) m ← Older edit		Revision as of 18:50, 13 October 2016 (view source) YangHongYu (Talk \| contribs) m Newer edit →
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	<partinfo>BBa_K2120311 short</partinfo>		<partinfo>BBa_K2120311 short</partinfo>
	pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And tetR is an inhibitor which is built to repress the expression of ptet promoter. We use it to control the “on” or ”off” state of toxin gene. RFP is the reporter gene. In order to test these do not interfere with each other , We reserve 【BBa_K2120303】 .		pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And tetR is an inhibitor which is built to repress the expression of ptet promoter. We use it to control the “on” or ”off” state of toxin gene. RFP is the reporter gene. In order to test these do not interfere with each other , We reserve 【BBa_K2120303】 .
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