Difference between revisions of "Part:BBa K2120310"

Revision as of 18:49, 13 October 2016

B0015+CI+B0034+pBAD-araC+B0015+pR+B0032+RFP+B0015 pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And CI is an inhibitor which is built to repress the expression of PR promoter. We use it to control the “on” or ”off” state of toxin gene.RFP is the reporter gene. In order to test these do not interfere with each other , We reserve 【BBa_K2120305】 . Compared with [BBa_K2120309],We add B0015 between AraC and pR

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1001
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1166
Illegal AgeI site found at 2925
Illegal AgeI site found at 3037
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1183

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2120310 short</partinfo>
-pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And CI is an inhibitor which is built to repress the expression of PR promoter. We use it to control the “on” or ”off” state of toxin gene.RFP is the reporter gene. In order to test these do not interfere with each other , We reserve 【BBa_K2120305】 .
+pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And CI is an inhibitor which is built to repress the expression of PR promoter. We use it to control the “on” or ”off” state of toxin gene.RFP is the reporter gene. In order to test these do not interfere with each other , We reserve 【BBa_K2120305】 . Compared with [BBa_K2120309],We add B0015 between AraC and pR