Difference between revisions of "Part:BBa K2120307"

Revision as of 18:29, 13 October 2016

B0015+tetR+B0034+pBAD-AraC+B0015 pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And tetR is an inhibitor which is built to repress the expression of Ptet promoter. We use it to control the “on” or ”off” state of toxin gene. In order to avoid leaking out, We reserve 【BBa_K2120303 】.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 911
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1076
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1093

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2120307 short</partinfo>
-pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And tetR is an inhibitor which is built to repress the expression of Ptet promoter. We use it to control the “on” or ”off” state of toxin gene. In order to avoid leaking out, We reserve it .
+pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And tetR is an inhibitor which is built to repress the expression of Ptet promoter. We use it to control the “on” or ”off” state of toxin gene. In order to avoid leaking out, We reserve  【BBa_K2120303 】.