Difference between revisions of "Part:BBa K1896008"

 
 
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<partinfo>BBa_K1896008 short</partinfo>
 
<partinfo>BBa_K1896008 short</partinfo>
  
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INP<sub>RC</sub> is a truncated variant of the ''inaZ'' from ''Pseudomonas syringae'' ([[Part:BBa_K1896001|Part:BBa_K1896001]]), the N-terminal domain was removed in order to prevent localisation to the outer membrane, while still maintaining ice nucleation activity. No stop codon is present in this part to allow seamless assembly of fusion proteins.
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INP<sub>RC</sub> is a truncated variant of the ''inaZ'' from ''Pseudomonas syringae'' ([[Part:BBa_K1896001|Part:BBa_K1896001]]). The N-terminal domain was removed in order to prevent localisation to the outer membrane [1], while still maintaining ice nucleation activity [2]. No stop codon is present in this part to allow seamless assembly of fusion proteins.
  
  
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<partinfo>BBa_K1896008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1896008 SequenceAndFeatures</partinfo>
  
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===References===
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# Li, L., Gyun Kang, D., &amp; Joon Cha, H. (2004). Functional display of foreign protein on surface of ''Escherichia coli'' using N‐terminal domain of ice nucleation protein. ''Biotechnology and bioengineering'', 85(2), 214-221.
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# Green, R. L., Corotto, L. V., &amp; Warren, G. J. (1988). Deletion mutagenesis of the ice nucleation gene from ''Pseudomonas syringae S203''. ''Molecular and General Genetics MGG'', 215(1), 165-172.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
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Latest revision as of 18:24, 13 October 2016


INP_RC

INPRC is a truncated variant of the inaZ from Pseudomonas syringae (Part:BBa_K1896001). The N-terminal domain was removed in order to prevent localisation to the outer membrane [1], while still maintaining ice nucleation activity [2]. No stop codon is present in this part to allow seamless assembly of fusion proteins.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 142
    Illegal NgoMIV site found at 1654
    Illegal NgoMIV site found at 1798
    Illegal NgoMIV site found at 2062
    Illegal NgoMIV site found at 2206
    Illegal NgoMIV site found at 2254
    Illegal NgoMIV site found at 2374
    Illegal AgeI site found at 1318
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  1. Li, L., Gyun Kang, D., & Joon Cha, H. (2004). Functional display of foreign protein on surface of Escherichia coli using N‐terminal domain of ice nucleation protein. Biotechnology and bioengineering, 85(2), 214-221.
  2. Green, R. L., Corotto, L. V., & Warren, G. J. (1988). Deletion mutagenesis of the ice nucleation gene from Pseudomonas syringae S203. Molecular and General Genetics MGG, 215(1), 165-172.