Difference between revisions of "Part:BBa K2120303"

 
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<partinfo>BBa_K2120303 short</partinfo>
 
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pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And tetR is an inhibitor which is built to repress the expression of Ptet promoter. We use it to control the “on” or ”off” state of toxin gene.
  
  

Revision as of 18:23, 13 October 2016


araC-pBAD+B0034+tetR+B0015 pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And tetR is an inhibitor which is built to repress the expression of Ptet promoter. We use it to control the “on” or ”off” state of toxin gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961