Difference between revisions of "Part:BBa K1957007"

 
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<partinfo>BBa_K1957007 short</partinfo>
 
<partinfo>BBa_K1957007 short</partinfo>
  
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One of the three subunits that make up NiFe Hydrogenase in Shewanella oneidensis. Specifically this is the HyaC subunit, which is the outer periplasmic subunit. This gene encodes one out of the three subunits that can be used to make the entire NiFe hydrogenase construct.
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One of the three subunits that make up NiFe Hydrogenase in Shewanella oneidensis. Specifically this is the HyaB subunit, which is the membrane-bound subunit. This subunit has a strep-II tag on its C-terminal for downstream protein assays. This gene encodes one out of the three subunits that can be used to make the entire NiFe hydrogenase construct.
  
  

Revision as of 17:47, 13 October 2016


HyaB C-terminally tagged subunit of NiFe Hydrogenase

One of the three subunits that make up NiFe Hydrogenase in Shewanella oneidensis. Specifically this is the HyaB subunit, which is the membrane-bound subunit. This subunit has a strep-II tag on its C-terminal for downstream protein assays. This gene encodes one out of the three subunits that can be used to make the entire NiFe hydrogenase construct.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 258
    Illegal BamHI site found at 1455
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1043
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1766