Difference between revisions of "Part:BBa K2110105"
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We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.
 
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.
 
https://static.igem.org/mediawiki/parts/thumb/f/f5/T--Tianjin--partdataS207T.png/800px-T--Tianjin--partdataS207T.png
 
https://static.igem.org/mediawiki/parts/thumb/f/f5/T--Tianjin--partdataS207T.png/800px-T--Tianjin--partdataS207T.png
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Actually, this is the mutant with the highest activity of degrading PET.
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<br/>Actually, this is the mutant with the highest activity of degrading PET.
  
 
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Revision as of 17:39, 13 October 2016


PETase site-directed mutant I208V

This is one of the site-directed mutant of the PETase gene, we change the 207th amino acid from S to T. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 79
    Illegal BglII site found at 289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Results

We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows. 800px-T--Tianjin--partdataS207T.png
Actually, this is the mutant with the highest activity of degrading PET.