Difference between revisions of "Part:BBa K1896005:Design"

 
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===References===
 
===References===
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# Lim, K. H., Huang, H., Pralle, A., & Park, S. (2013). Stable, high‐affinity streptavidin monomer for protein labeling and monovalent biotin detection. ''Biotechnology and bioengineering'', 110(1), 57-67.

Latest revision as of 17:28, 13 October 2016


mSA2-His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 97
    Illegal AgeI site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is not compatible with the Freiburg assembly standard (RFC 25).


Source

Combination of streptavidin (Streptomyces avidinii) and rhizavidin (Rhizobium etli), engineered through homology modeling [1].

References

  1. Lim, K. H., Huang, H., Pralle, A., & Park, S. (2013). Stable, high‐affinity streptavidin monomer for protein labeling and monovalent biotin detection. Biotechnology and bioengineering, 110(1), 57-67.