Difference between revisions of "Part:BBa K1937004"

 
Line 5: Line 5:
 
(Chassis B. subtilis, carrier plasmid pBS0K)
 
(Chassis B. subtilis, carrier plasmid pBS0K)
 
Length: 6843 bp
 
Length: 6843 bp
−
Background:
+
 
 +
==Background:==
 
pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in Bacillus subtilis.
 
pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in Bacillus subtilis.
 
In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate. This NAG molecule is the major component of chitin, a characteristic molecule of fungi.  
 
In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate. This NAG molecule is the major component of chitin, a characteristic molecule of fungi.  

Revision as of 17:19, 13 October 2016


pNagA-RFP in pSBBs0K-mini plasmid

(Chassis B. subtilis, carrier plasmid pBS0K) Length: 6843 bp

Background:

pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in Bacillus subtilis. In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate. This NAG molecule is the major component of chitin, a characteristic molecule of fungi. This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1257
    Illegal suffix found in sequence at 1285
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1257
    Illegal NheI site found at 201
    Illegal NheI site found at 1279
    Illegal SpeI site found at 1286
    Illegal PstI site found at 1300
    Illegal NotI site found at 1263
    Illegal NotI site found at 1293
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1257
    Illegal BglII site found at 999
    Illegal BglII site found at 5814
    Illegal BamHI site found at 1330
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1257
    Illegal suffix found in sequence at 1286
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1257
    Illegal XbaI site found at 1272
    Illegal SpeI site found at 1286
    Illegal PstI site found at 1300
    Illegal NgoMIV site found at 2877
    Illegal AgeI site found at 761
    Illegal AgeI site found at 873
    Illegal AgeI site found at 5425
    Illegal AgeI site found at 6387
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1055
    Illegal BsaI.rc site found at 2701
    Illegal BsaI.rc site found at 4140
    Illegal BsaI.rc site found at 6656
    Illegal SapI site found at 1618
    Illegal SapI.rc site found at 5638