Difference between revisions of "Part:BBa K2110006"

 
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
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<partinfo>BBa_K2110006 SequenceAndFeatures</partinfo>
  

Latest revision as of 17:06, 13 October 2016


TPA regulatory gene expression system

This part consists of the enhanced promoter PGK1(BBa_K2110012), the tpaR gene(BBa_K2110002), and the terminator CYC1（BBa_K2110000）. The tpaR gene encodes the terephthalic acid (TPA) positive feedback regulatory protein. This protein can bind the TPA inducible leader sequence (TILS) at the existence of TPA and induce the expression of downstream gene. The tpaR protein belongs to the IclR protein family and has helix-turn-helix domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1581
    Illegal AgeI site found at 1259
    Illegal AgeI site found at 1453
  • 1000
    COMPATIBLE WITH RFC[1000]