Difference between revisions of "Part:BBa K1935001:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Because DSIP sequence is very short, we decided to put a P2A sequence between two DSIP sequences.
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Nucleic sequence and coding gene of DSIP is unknown. We decide to convert amino-acid sequence into nucleic sequence.
−
 
+
After that we used optimisation codon sequence for Caenorhabditis elegans.
−
 
+
We decided to add two DSIP sequence with P2A sequence between them.
 +
We also added an ATG codon stop in 5'-end.
  
 
===Source===
 
===Source===

Revision as of 14:00, 13 October 2016


DSIP - delta sleep inducing peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nucleic sequence and coding gene of DSIP is unknown. We decide to convert amino-acid sequence into nucleic sequence. After that we used optimisation codon sequence for Caenorhabditis elegans. We decided to add two DSIP sequence with P2A sequence between them. We also added an ATG codon stop in 5'-end.

Source

Delta sleep-inducing peptide (DSIP): a still unresolved riddle". Journal of Neurochemistry Kovalzon VM and Strekalova TV (2006)

Delta-Sleep-Inducing Peptide (DSIP): A Review MARKUS V. GRAF AND ABBA J.KASTIN

References