Difference between revisions of "Part:BBa K1935009:Design"

 
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===References===
 
===References===
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http://www.wormbase.org/species/c_elegans/gene/WBGene00003515#01-9g-3

Latest revision as of 13:31, 13 October 2016


pMYO-3 : Specific muscle promoter of C.elegans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 53
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1446


Design Notes

Mutagenesis was done to abolish three illegal restriction sites : SpeI (1018-1023), PstI (1212-1217) and XbaI (2025-2030).



Source

PROMOTEROME : http://worfdb.dfci.harvard.edu/promoteromedb/searchPromoterome.pl?by=name&sid=myo-3

References

http://www.wormbase.org/species/c_elegans/gene/WBGene00003515#01-9g-3