Difference between revisions of "Part:BBa K1935005:Design"

Latest revision as of 12:41, 13 October 2016

White collar-2 (WC-2) - photoreceptor in Neurospora tetrasperma

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 25
Illegal BamHI site found at 61
Illegal BamHI site found at 835
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1251
Illegal NgoMIV site found at 1310
Illegal AgeI site found at 857
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 402
Illegal BsaI.rc site found at 770
Illegal SapI.rc site found at 652

A mutagenesis was done to abolish an illegal restriction site (Pst1 : 425 to 430 pb, CTGCAG => CAGCAG)

Revision as of 12:33, 13 October 2016 (view source) Registry (Talk \| contribs)		Latest revision as of 12:41, 13 October 2016 (view source) EmiG (Talk \| contribs)
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	===Design Notes===		===Design Notes===
−	A mutagenesis was done to abolish an illegal restriction site (~~PST1~~)	+	A mutagenesis was done to abolish an illegal restriction site (Pst1 : 425 to 430 pb, CTGCAG => CAGCAG)