Difference between revisions of "Part:BBa K1937003:Experience"
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===Applications of BBa_K1937003=== | ===Applications of BBa_K1937003=== | ||
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| + | The part was sub-cloned in thepSBBsOK-mini plasmid (BBa_K1937001) for validation (resulting in part BBa_K1937004).In presence of N-acetyl glucosamine in the media, the clones are expected to be red, and white without this substrate Nag. | ||
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| + | BBa_K1937003-results.png | ||
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| + | We indeed observed some red stains on the colonies only with the pNagA promoter growing on NAG (as well as with the pNagP promoter, see part BBa_K1937006), but the expression is late during the colonies development and does not seem to be specific (same result with the control plate on glucose) | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 12:39, 13 October 2016
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1937003
The part was sub-cloned in thepSBBsOK-mini plasmid (BBa_K1937001) for validation (resulting in part BBa_K1937004).In presence of N-acetyl glucosamine in the media, the clones are expected to be red, and white without this substrate Nag.
BBa_K1937003-results.png
We indeed observed some red stains on the colonies only with the pNagA promoter growing on NAG (as well as with the pNagP promoter, see part BBa_K1937006), but the expression is late during the colonies development and does not seem to be specific (same result with the control plate on glucose)
User Reviews
UNIQ0ecf79473c09bca0-partinfo-00000000-QINU UNIQ0ecf79473c09bca0-partinfo-00000001-QINU