Difference between revisions of "Part:BBa K1937003"
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__NOTOC__ <partinfo>BBa_K1937003 short</partinfo> The BBa_K1937003 part was designed to demonstrate the induction of pNagA by NAG. It belongs tothe antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France). pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in Bacillus subtilis In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6 phosphate. This NAG molecule is the major component of chitin, a characteristic molecule of fungi. <!-- Add more about the biology of this part here ===Usage and Biology=== <!-- --> <span class='h3bb'>Sequence and Features</span> <partinfo>BBa_K1937003 SequenceAndFeatures</partinfo> <!-- Uncomment this to enable Functional Parameter display ===Functional Parameters=== <partinfo>BBa_K1937003 parameters</partinfo> <!-- -->
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__NOTOC__
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<partinfo>BBa_K1364000 short</partinfo>
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<br> This part is designed to enable a''' N-acetylatedglucosamine based chemotaxis''' in ''Bacillus subtilis''.
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<br>'''Design''' :<br>
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The encoded protein is a chimera of 2 proteins:<br>
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-the '''methyl accepting Chemotaxis protein''' (McpA, http://www.uniprot.org/uniprot/P39214) from ''Bacillus subtilis'' which is required for taxis towards glucose, <br>
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-and the '''N-acetylglucosamine regulated methyl-accepting chemotaxis protein''' from ''Vibrio cholerae'' (VCD_000306, http://www.uniprot.org/uniprot/C3NYT2).
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The chimeric protein contains the intracellular domains of McpA from B''acillus subtilis'' to enable the transduction of the signal: these domains correspond to the amino acids 1-37 and 282-610. The extracellular domain of VCD_000306 is inserted between the intracellular regions of McpA to sense N-acetylglucosamine and corresponds to the amino acids  31-310.
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https://static.igem.org/mediawiki/parts/d/db/Chemotaxis_part.jpg
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'><b>Sequence and Features</b></span>
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<partinfo>BBa_K1364000 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
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===Functional Parameters===
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<partinfo>BBa_K1364000 parameters</partinfo>
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<!-- -->
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'''References''' : <br>
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K. Meibom,L. Xibing, A. Nielsen, CY. Wu, S. Roseman and G. Schoolnik.<b> The Vibrio cholerae chitin utilization program </b>. The National Academy of Sciences of the USA (2004).
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C. Kristich and GW. Ordal.<b>Bacillus subtilis CheD is a chemoreceptor modification enzyme required for chemotaxis</b>. J Biol Chem. 2002 Jul 12;277(28):25356-62. Epub 2002 May 13.
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Revision as of 12:28, 13 October 2016

pNagA-RFP The BBa_K1937003 part was designed to demonstrate the induction of pNagA by NAG. It belongs tothe antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France). pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in Bacillus subtilis In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6 phosphate. This NAG molecule is the major component of chitin, a characteristic molecule of fungi.  Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 201
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 761
    Illegal AgeI site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]