Difference between revisions of "Part:BBa K1937003"
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__NOTOC__ | __NOTOC__ | ||
− | <partinfo> | + | <partinfo>BBa_K1364000 short</partinfo> |
− | + | <br> This part is designed to enable a''' N-acetylatedglucosamine based chemotaxis''' in ''Bacillus subtilis''. | |
− | + | ||
− | + | ||
− | + | ||
+ | <br>'''Design''' :<br> | ||
+ | The encoded protein is a chimera of 2 proteins:<br> | ||
+ | -the '''methyl accepting Chemotaxis protein''' (McpA, http://www.uniprot.org/uniprot/P39214) from ''Bacillus subtilis'' which is required for taxis towards glucose, <br> | ||
+ | -and the '''N-acetylglucosamine regulated methyl-accepting chemotaxis protein''' from ''Vibrio cholerae'' (VCD_000306, http://www.uniprot.org/uniprot/C3NYT2). | ||
+ | The chimeric protein contains the intracellular domains of McpA from B''acillus subtilis'' to enable the transduction of the signal: these domains correspond to the amino acids 1-37 and 282-610. The extracellular domain of VCD_000306 is inserted between the intracellular regions of McpA to sense N-acetylglucosamine and corresponds to the amino acids 31-310. | ||
+ | |||
+ | https://static.igem.org/mediawiki/parts/d/db/Chemotaxis_part.jpg | ||
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<!-- --> | <!-- --> | ||
− | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><b>Sequence and Features</b></span> |
− | <partinfo> | + | <partinfo>BBa_K1364000 SequenceAndFeatures</partinfo> |
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
− | <partinfo> | + | <partinfo>BBa_K1364000 parameters</partinfo> |
<!-- --> | <!-- --> | ||
+ | |||
+ | '''References''' : <br> | ||
+ | K. Meibom,L. Xibing, A. Nielsen, CY. Wu, S. Roseman and G. Schoolnik.<b> The Vibrio cholerae chitin utilization program </b>. The National Academy of Sciences of the USA (2004). | ||
+ | |||
+ | C. Kristich and GW. Ordal.<b>Bacillus subtilis CheD is a chemoreceptor modification enzyme required for chemotaxis</b>. J Biol Chem. 2002 Jul 12;277(28):25356-62. Epub 2002 May 13. |
Revision as of 12:27, 13 October 2016
N-acetylatedGlucosamine based chemotaxis for Bacillus subtilis
This part is designed to enable a N-acetylatedglucosamine based chemotaxis in Bacillus subtilis.
Design :
The encoded protein is a chimera of 2 proteins:
-the methyl accepting Chemotaxis protein (McpA, http://www.uniprot.org/uniprot/P39214) from Bacillus subtilis which is required for taxis towards glucose,
-and the N-acetylglucosamine regulated methyl-accepting chemotaxis protein from Vibrio cholerae (VCD_000306, http://www.uniprot.org/uniprot/C3NYT2).
The chimeric protein contains the intracellular domains of McpA from Bacillus subtilis to enable the transduction of the signal: these domains correspond to the amino acids 1-37 and 282-610. The extracellular domain of VCD_000306 is inserted between the intracellular regions of McpA to sense N-acetylglucosamine and corresponds to the amino acids 31-310.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1913
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1370
Illegal SapI.rc site found at 2006
References :
K. Meibom,L. Xibing, A. Nielsen, CY. Wu, S. Roseman and G. Schoolnik. The Vibrio cholerae chitin utilization program . The National Academy of Sciences of the USA (2004).
C. Kristich and GW. Ordal.Bacillus subtilis CheD is a chemoreceptor modification enzyme required for chemotaxis. J Biol Chem. 2002 Jul 12;277(28):25356-62. Epub 2002 May 13.