Difference between revisions of "Part:BBa K2150110"
| Line 3: | Line 3: | ||
<partinfo>BBa_K2150110 short</partinfo> | <partinfo>BBa_K2150110 short</partinfo> | ||
| − | a | + | This composite part consists of LacI under constitutive promoter, and toxin gene of toxin N-acetyltransferase GCN5 (In our project, ID: 1204) under pTac prompter. |
| + | Directly upstream of coding region of toxin gene is RiboJ, an insulator buffers gene expression from its cellular context. RIboJ is a ribozyme which effetely cleaves 5'-UTR of mRNAs. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | We used this device to test the toxicity of toxin protein. Different amount of IPTG is added, and the growth curve of bacteria is measured using 96-well microplate spectrophotometer. | |
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 08:37, 13 October 2016
LacI (constitutive) + Ptac + RiboJ + 1204 toxin
This composite part consists of LacI under constitutive promoter, and toxin gene of toxin N-acetyltransferase GCN5 (In our project, ID: 1204) under pTac prompter. Directly upstream of coding region of toxin gene is RiboJ, an insulator buffers gene expression from its cellular context. RIboJ is a ribozyme which effetely cleaves 5'-UTR of mRNAs.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1611
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1857
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1611
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1611
Illegal NgoMIV site found at 1788 - 1000COMPATIBLE WITH RFC[1000]