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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 03:33, 13 October 2016


LacZ

A cell wall binding domain used in surface display for L. lactis

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

To build a surface display system, the first thing should be considered is the cell wall anchoring motif. The most exploited anchoring regions are those with the LPXTG motifs that bind the proteins in a covalent way to the cell wall. However, to achieve versatile application, it is better to use anchoring domains that interact with the cell wall in a non-covalent way. The major autolysin of L. lactis, the cell wall hydrolase AcmA contains 3 tandem arranged LysM motifs and separated by stretches of 21 to 31 amino acids, this region is collectively termed as the cA anchoring domain. The cA domain can be fused to the N- and C- terminus of functional proteins, and can bind proteins to the cell walls of a broad range of gram-positive bacteria.

References

[1] Buist, G., Steen, A., Kok, J., and Kuipers, O.P. (2008). LysM, a widely distributed protein motif for binding to (peptido)glycans. Mol Microbiol 68, 838-847.

[2] Raha, A.R., Varma, N.R., Yusoff, K., Ross, E., and Foo, H.L. (2005). Cell surface display system for Lactococcus lactis: a novel development for oral vaccine. Appl Microbiol Biotechnol 68, 75-81.