Difference between revisions of "Part:BBa K2150022:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
T7RNAP:PCR amplified from genome of BL21
+
T7RNAP:PCR amplified from genome of BL21.
 +
 
 
First terminator comes from BBa_B0013, while the second terminator from BBa_B0015.
 
First terminator comes from BBa_B0013, while the second terminator from BBa_B0015.
 
PTet from BBa_R0040,RBS from BBa_B0034, pT7 from BBa_K525998, GFP from BBa_E0040.
 
PTet from BBa_R0040,RBS from BBa_B0034, pT7 from BBa_K525998, GFP from BBa_E0040.
  
 
===References===
 
===References===

Revision as of 03:24, 13 October 2016


pTet+RBS+T7RNAP+Ter+pT7+RBS+GFP+DT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3488


Design Notes

This part is used to test the positive feedback effect of T7RNAP and pT7.


Source

T7RNAP:PCR amplified from genome of BL21.

First terminator comes from BBa_B0013, while the second terminator from BBa_B0015. PTet from BBa_R0040,RBS from BBa_B0034, pT7 from BBa_K525998, GFP from BBa_E0040.

References