Difference between revisions of "Part:BBa K2150021"

Revision as of 03:18, 13 October 2016

pCon+RBS+tetR+DT+pT7+pTet+RBS+GFP+RBS+T7RNAP+DT

This part is a complete regulatory device sensitive to tetracycline. The first promoter is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no fluorescence can be detected. In the presence of Tc, however, tetR can be repressed by Tc and repression over pTet can be removed, resulting in the expression of GFP and T7RNAP. Under such circumstances, positive feedback can be achieved and obvious fluorescence can be observed. This part is used to test the tet switch and the tet-on/tet-off system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1643

@@ Line 4: / Line 4: @@
 This part is a complete regulatory device sensitive to tetracycline.
-The first promoter is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no fluorescence can be detected. In the presence of Tc, however, tetR can be repressed be Tc and repression over pTet can be removed, resulting in the expression of GFP and T7RNAP. Under such circumstances, positive feedback can be achieved and obvious fluorescence can be  observed. This part is used to test the tet switch and the tet-on/tet-off system.
+The first promoter is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no fluorescence can be detected. In the presence of Tc, however, tetR can be repressed by Tc and repression over pTet can be removed, resulting in the expression of GFP and T7RNAP. Under such circumstances, positive feedback can be achieved and obvious fluorescence can be  observed. This part is used to test the tet switch and the tet-on/tet-off system.
 <!-- Add more about the biology of this part here