Difference between revisions of "Part:BBa R0040"
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Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, [http://openwetware.org/wiki/ATc aTc]. | Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, [http://openwetware.org/wiki/ATc aTc]. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 01:53, 13 October 2016
TetR repressible promoter
Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, [http://openwetware.org/wiki/ATc aTc].
Usage and Biology
Usage and Biology
Medium strength promoter. [jb, 5/24/04]
From the reference article:"In contrast to tetracycline, anhydrotetracycline is a particularly useful inducer. It binds Tet R with an ~35-fold higher binding constant and thus allows to operate at very low concentrations. At the same time, its antibiotic activity is ~100-fold lower and concentrations of <50 ng/ml as required for the full induction of P LtetO-1 have no effect on the growth of E.coli."
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]