Difference between revisions of "Part:BBa K1969020"

 
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<partinfo>BBa_K1969020 short</partinfo>
 
<partinfo>BBa_K1969020 short</partinfo>
  
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It is the linker sequence between ADH1 terminator and ADRB2 downstream GAL1 promoter.
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The sequence of ADRB2 with a His-tag at its C terminus is cloned downstream the yeast ADH1 promoter and ended with an ADH1 terminator to generate a expression cassette in yeast Saccharomyces cerevisiae.
  
 
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Revision as of 01:06, 13 October 2016


ADRB2 with His-tag regulated by ADH1 promoter

The sequence of ADRB2 with a His-tag at its C terminus is cloned downstream the yeast ADH1 promoter and ended with an ADH1 terminator to generate a expression cassette in yeast Saccharomyces cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1522
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 90
    Illegal BsaI.rc site found at 1108
    Illegal BsaI.rc site found at 1306