Difference between revisions of "Part:BBa K2019001"
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<partinfo>BBa_K2019001 short</partinfo>
 
<partinfo>BBa_K2019001 short</partinfo>
  
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Part BBa_K2019001 is a part consisting of part BBa_J04450 followed by BBa_J23119-Guide Template-gRNA Scaffolding downstream.  This part has a customizable guide template, which is flanked on both ends by BsaI sites.
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Part BBa_K2019001 is a part consisting of part BBa_J04450 followed by BBa_J23119-Guide Template-gRNA Scaffolding downstream.  This part has a customizable guide template, which is flanked on both ends by BsaI sites to allow for a new guide to be inserted in place of the template through Golden Gate Assembly.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:58, 12 October 2016


mRFP+gRNA Template (for saCas9)

Part BBa_K2019001 is a part consisting of part BBa_J04450 followed by BBa_J23119-Guide Template-gRNA Scaffolding downstream. This part has a customizable guide template, which is flanked on both ends by BsaI sites to allow for a new guide to be inserted in place of the template through Golden Gate Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1099
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1076
    Illegal SpeI site found at 1099
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1099
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1099
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1154
    Illegal BsaI.rc site found at 1106