Difference between revisions of "Part:BBa K2042014:Design"

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===Design Notes===
 
===Design Notes===
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None
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We inserted non-coding scar sequences after RBS to prevent missing initial codons during translation.
  
 
===Source===
 
===Source===

Revision as of 22:09, 12 October 2016


RBS + PhaC (sp. MBEL 6-19) + RBS + PCT (CP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2792
    Illegal BsaI site found at 3263


Design Notes

We inserted non-coding scar sequences after RBS to prevent missing initial codons during translation.

Source

The RBS were synthesized from the genomic sequence of P.putida, Pct were synthetised from genomic sequence of Clostridum propionicum and PhaC from Pseudomonas sp. MBEL 6-19

References