Difference between revisions of "Part:BBa K2042014:Design"

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===Source===
 
===Source===
  
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None
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The RBS were synthesized from the genomic sequence of P.putida, Pct were synthetised from genomic sequence of Clostridum propionicum and PhaC from Pseudomonas sp. MBEL 6-19
  
 
===References===
 
===References===

Revision as of 22:07, 12 October 2016


RBS + PhaC (sp. MBEL 6-19) + RBS + PCT (CP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2386
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
    Illegal NgoMIV site found at 1352
    Illegal AgeI site found at 148
    Illegal AgeI site found at 461
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2792
    Illegal BsaI site found at 3263


Design Notes

None

Source

The RBS were synthesized from the genomic sequence of P.putida, Pct were synthetised from genomic sequence of Clostridum propionicum and PhaC from Pseudomonas sp. MBEL 6-19

References