Difference between revisions of "Part:BBa K2066506"

Latest revision as of 18:29, 12 October 2016

RiboJ (Ribozyme Insulator) Lou et. al. 2012

RiboJ is the sequence for a ribozyme studied in Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context"). WM iGEM 2016 used this sequence between the promoter and ribosome sequence. One of our goals for using this part is moving it onto a Biobrick backbone. Furthermore, In Lou et. al, this ribozyme sequence was said to act as an insulator which generalizes protein expression levels for a given promoter. We used RiboJ to collect data for our Ribozyme characterization project as well as our ribosome and promoter characterization projects. The last six base pairs of the sequence is a scar"ACTAGA" which Lou et al. had in their sequence which we conserved throughout all of our uses of RiboJ from the 2016 WM iGEM season.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2066506 short</partinfo>
-RiboJ is the sequence for a ribozyme studied in Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context").  WM iGEM 2016 used this sequence between the promoter and ribosome sequence.  One of our goals for using this part is moving it onto a Biobrick backbone.  Furthermore, In Lou et. al, this ribozyme sequence was said to act as an insulator which generalizes protein expression levels for a given promoter. We used RiboJ to collect data for our Ribozyme characterization project as well as our ribosome and promoter characterization projects.
+RiboJ is the sequence for a ribozyme studied in Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context").  WM iGEM 2016 used this sequence between the promoter and ribosome sequence.  One of our goals for using this part is moving it onto a Biobrick backbone.  Furthermore, In Lou et. al, this ribozyme sequence was said to act as an insulator which generalizes protein expression levels for a given promoter. We used RiboJ to collect data for our Ribozyme characterization project as well as our ribosome and promoter characterization projects. The last six base pairs of the sequence is a scar"ACTAGA" which Lou et al. had in their sequence which we conserved throughout all of our uses of RiboJ from the 2016 WM iGEM season.
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