Difference between revisions of "Part:BBa K2110100"
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<partinfo>BBa_K2110100 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2110100 SequenceAndFeatures</partinfo> | ||
| − | + | Activity Assay<br/> | |
| + | We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows. | ||
| + | https://static.igem.org/mediawiki/parts/e/e6/T--Tianjin--partA240P.png | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2110100 parameters</partinfo> | <partinfo>BBa_K2110100 parameters</partinfo> | ||
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Revision as of 14:45, 12 October 2016
PETase site-directed mutant A240P
This is one of the site-directed mutant of the PETase gene, we change the 240th amino acid from A to P. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 79
Illegal BglII site found at 289 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Activity Assay
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.
