Difference between revisions of "Part:BBa K1921013"

 
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<partinfo>BBa_K1921013 short</partinfo>
 
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<partinfo>BBa_K1921013 parameters</partinfo>
 
<partinfo>BBa_K1921013 parameters</partinfo>
 
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===Usage===
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The discovery of the autotransporter family has provided a mechanism for surface expression of proteins in laboratory strains of Escherichia coli. AIDA is one of the members of autotransporter family. We call it one of an anchor proteins for it can immobilize on the outer membrane of Escherichia coli. It is very useful because we can insert other proteins’ sequence into the AIDA sequence, and then the protein will be immobilized on the outer membrane. We can use it to do whole cell catalysis. In addition, it also have a broad range of applications in molecular biology, biochemistry, biotechnology, microbiology and vaccinology . Today we use this part to display our PETase on E.coli’s surface. Compared with the eukaryotic surface display system, display system with surface expression in prokaryotes cycle is short. In addition, prokaryote surface display system method is simple and mature. AIDAc is the autotransporter adhesin involved in diffuse adherence, so it can anchor stably on the membrane.
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===Biology===
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Surface expression of recombinant proteins was first described more than 30 years ago. AIDA is one of an anchor proteins which belongs to Escherichia coli(Escherichia coli strain 2787). We find its sequence from NCBI. Surface display using AIDA contains three parts: signal peptide, passenger domain and anchor protein AIDAc. So this is a C-terminal anchoring. AIDAc protein structure has been analyzed. It is a transmembrane protein across the 12 membrane and its shape is just like a β-barrel. Theβ-barrel can be anchored on the outer membrane so that the special protein can be displayed on the surface.
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===Reference===
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[1] Jarmander, Johan; Gustavsson, Martin; Thi-Huyen Do: A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli. MICROBIAL CELL FACTORIES 2012,11

Revision as of 14:21, 12 October 2016


INPN


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 72
    Illegal NgoMIV site found at 405
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage

The discovery of the autotransporter family has provided a mechanism for surface expression of proteins in laboratory strains of Escherichia coli. AIDA is one of the members of autotransporter family. We call it one of an anchor proteins for it can immobilize on the outer membrane of Escherichia coli. It is very useful because we can insert other proteins’ sequence into the AIDA sequence, and then the protein will be immobilized on the outer membrane. We can use it to do whole cell catalysis. In addition, it also have a broad range of applications in molecular biology, biochemistry, biotechnology, microbiology and vaccinology . Today we use this part to display our PETase on E.coli’s surface. Compared with the eukaryotic surface display system, display system with surface expression in prokaryotes cycle is short. In addition, prokaryote surface display system method is simple and mature. AIDAc is the autotransporter adhesin involved in diffuse adherence, so it can anchor stably on the membrane.

Biology

Surface expression of recombinant proteins was first described more than 30 years ago. AIDA is one of an anchor proteins which belongs to Escherichia coli(Escherichia coli strain 2787). We find its sequence from NCBI. Surface display using AIDA contains three parts: signal peptide, passenger domain and anchor protein AIDAc. So this is a C-terminal anchoring. AIDAc protein structure has been analyzed. It is a transmembrane protein across the 12 membrane and its shape is just like a β-barrel. Theβ-barrel can be anchored on the outer membrane so that the special protein can be displayed on the surface.

Reference

[1] Jarmander, Johan; Gustavsson, Martin; Thi-Huyen Do: A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli. MICROBIAL CELL FACTORIES 2012,11