Difference between revisions of "Part:BBa K2040101"

 
Line 6: Line 6:
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 +
 
===Usage and Biology===
 
===Usage and Biology===
  
 +
The minimal PgpdA is from
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 13:54, 12 October 2016


Fungal promoter PgpdA

PgpdA is the Aspergillus niger glyceraldehyde-3-phosphate dehydrogenase promoter. This 2.1kb promoter containing a minimal gpdA promoter(BBa_K2040200) from A. nidulans was isolated from vector pAN7-1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 903
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 137
    Illegal XhoI site found at 693
    Illegal XhoI site found at 1075
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
    Illegal AgeI site found at 1430
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1109
    Illegal SapI site found at 315