Difference between revisions of "Part:BBa K1884001"

Revision as of 08:38, 12 October 2016

Gal4BD-CIB1

Cryptochrome-interacting basic-helix-loop-helix 1(CIB1) is a protein-coded gene. The product of this gene expression is a basic helix-loop-helix (bHLH) protein which would interact with cryptochrome 2 (CRY2), a blue light stimulated photoreceptor, when exposed to blue light. This part is a Gal4 DNA binding domain fused to N terminus of CIB1

Usage and Biology

This fusion protein is for use in a yeast-two-hybrid system,and a Gal4 DNA bindin domian fused to its N terminus

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 218
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 637
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 137

@@ Line 5: / Line 5: @@
 Cryptochrome-interacting basic-helix-loop-helix 1(CIB1) is a protein-coded gene. The product of this gene expression is a basic helix-loop-helix (bHLH) protein which would interact with cryptochrome 2 (CRY2), a blue light stimulated photoreceptor, when exposed to blue light. This part is a Gal4 DNA binding domain fused to N terminus of CIB1
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-This fusion protein is for use in a yeast-two-hybrid system,and a Gal4 DNA bindin domian fused to its N terminus.
+This fusion protein is for use in a yeast-two-hybrid system,and a Gal4 DNA bindin domian fused to its N terminus
+<!-- Add more about the biology of this part here
+===Usage and Biology===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>