Difference between revisions of "Part:BBa K1949030:Design"
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===Source=== | ===Source=== | ||
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Revision as of 06:51, 12 October 2016
yafO
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 335
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
yafO gene fragment amplified by PCR contains XbaI site at N-terminal and SpeI and PstI site at C-terminal. Both terminals were cut by respective restriction enzymes and the fragment used for ligation with iGEM plasmid vectors.
fwd:5’-AAATCTAGATGCGGGTATTCAAAACAAAACTTA-3’
rev:5’-AAACTGCAGCGGCCGCTACTAGTATTATTAAAAACGCATGCGAAACGCTTCTGCCA-3’
Source
The gene sequence was isolated from E. coli BL21
===References===