Difference between revisions of "Part:BBa K1949030:Design"

Line 12: Line 12:
 
rev:5’-AAACTGCAGCGGCCGCTACTAGTATTATTAAAAACGCATGCGAAACGCTTCTGCCA-3’
 
rev:5’-AAACTGCAGCGGCCGCTACTAGTATTATTAAAAACGCATGCGAAACGCTTCTGCCA-3’
 
   
 
   
 
 
  
 
===Source===
 
===Source===
  
This part is derived from E.coli,BM26, amplified by using PCR.
+
The gene sequence was isolated from E. coli BL21===References===
 
+
 
+
===References===
+

Revision as of 06:51, 12 October 2016


yafO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 335
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

yafO gene fragment amplified by PCR contains XbaI site at N-terminal and SpeI and PstI site at C-terminal. Both terminals were cut by respective restriction enzymes and the fragment used for ligation with iGEM plasmid vectors.

fwd:5’-AAATCTAGATGCGGGTATTCAAAACAAAACTTA-3’
rev:5’-AAACTGCAGCGGCCGCTACTAGTATTATTAAAAACGCATGCGAAACGCTTCTGCCA-3’


Source

The gene sequence was isolated from E. coli BL21===References===