Difference between revisions of "Part:BBa K1949030:Design"
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<span style="margin-left: 10px;"><i>yafO</i> gene fragment amplified by PCR contains <i>Xba</i>I site at N-terminal and <i>Spe</i>I and <i>Pst</i>I site at C-terminal. Both terminals were cut by respective restriction enzymes and the fragment used for ligation with iGEM plasmid vectors.<br>
 
<span style="margin-left: 10px;"><i>yafO</i> gene fragment amplified by PCR contains <i>Xba</i>I site at N-terminal and <i>Spe</i>I and <i>Pst</i>I site at C-terminal. Both terminals were cut by respective restriction enzymes and the fragment used for ligation with iGEM plasmid vectors.<br>
  
−
fwd:5’-AAATCTAGATGCGGGTATTCAAAACAAAACTTA-3’
+
fwd:5’-AAATCTAGATGCGGGTATTCAAAACAAAACTTA-3’<br>
 
rev:5’-AAACTGCAGCGGCCGCTACTAGTATTATTAAAAACGCATGCGAAACGCTTCTGCCA-3’
 
rev:5’-AAACTGCAGCGGCCGCTACTAGTATTATTAAAAACGCATGCGAAACGCTTCTGCCA-3’
 
   
 
   

Revision as of 06:50, 12 October 2016


yafO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 335
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

yafO gene fragment amplified by PCR contains XbaI site at N-terminal and SpeI and PstI site at C-terminal. Both terminals were cut by respective restriction enzymes and the fragment used for ligation with iGEM plasmid vectors.

fwd:5’-AAATCTAGATGCGGGTATTCAAAACAAAACTTA-3’
rev:5’-AAACTGCAGCGGCCGCTACTAGTATTATTAAAAACGCATGCGAAACGCTTCTGCCA-3’



Source

This part is derived from E.coli,BM26, amplified by using PCR.


===References===