Difference between revisions of "Part:BBa K1940001"
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==Sequence and Features== | ==Sequence and Features== | ||
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== References == | == References == | ||
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Revision as of 05:41, 12 October 2016
We designed this part to detect the abundance of microRNA-34c. Anti-microRNA-34c ( BBa_K1940000) was insert after a constitutive promoter (BBa_J23106) which express GFP. As anti- microRNA-34c is sensitive to the expression levels of miR-34c, we would have a direct impact on GFP’s expression. Determining GFP’s fluorescence intensity differences will justify the content amount of microRNA-34c.
Sequence and Features
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Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 186
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 50
Illegal BsaI.rc site found at 183
Illegal BsaI.rc site found at 864
References