Difference between revisions of "Part:BBa K2089004"

Revision as of 05:10, 12 October 2016

pHHL from Arabidopsis

pHhl1 is the promoter of gene hhl1 in wild type Arabidopsis,the promoter may be regulated by the concentration of certain protein differs from before and after photodamage takes place in photosystemⅡ.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 456
Illegal SpeI site found at 1469
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 910
Illegal SpeI site found at 456
Illegal SpeI site found at 1469
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 456
Illegal SpeI site found at 1469
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 456
Illegal SpeI site found at 1469
Illegal NgoMIV site found at 706
1000
COMPATIBLE WITH RFC[1000]

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 <!-- Add more about the biology of this part here
-In order to examine the efficiency of this promoter,we made a part which consists of a complete functional protein HHL1 coding sequence in Arabidopsis and gene hhl1,and this part has been taken into the nucleus of protoplasts of Arabiodopsis using psb1c3 plasmid as its carrier.RT-PCR is done to the transcribed mRNA of gene hhl1 to get cDNA,then we use the ultraviolet spectrophotometer to measure the concentration of cDNA we got.The concentration data is used to calculate the relative expression level of gene hhl1 compared to the gene expression of gene hhl1 regulated by the promoter pif1,cop1 and phyB.
-From the result,this part has no significent change in expression level of mRNA in growth light(100lux) and in high light(1200lux).This means that the promoter pHhl1 is repressed in these light intensities so the expression level is quite low.
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