Difference between revisions of "Part:BBa K1887000"
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===Usage and Biology===
 
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The lacZ gene from Lactobacillus acidophilus was introduced in the His locus of L. lactis NZ9000 genome by temperature sensitive vector, which makes the NZ-Blue strains become blue on plate with X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside).
 
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1887000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1887000 SequenceAndFeatures</partinfo>
  
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===Usage and Biology===
 +
The lacZ gene from Lactobacillus acidophilus was introduced in the His locus of L. lactis NZ9000 genome by temperature sensitive vector, which makes the NZ-Blue strains become blue on plate with X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside).
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===References===
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Simoes-Barbosa, A., Abreu, H., Silva Neto, A., Gruss, A., and Langella, P. (2004). A food-grade delivery system for Lactococcus lactis and evaluation of inducible gene expression. Appl Microbiol Biotechnol 65, 61-67.
  
 
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Revision as of 03:59, 12 October 2016


LacZ


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

The lacZ gene from Lactobacillus acidophilus was introduced in the His locus of L. lactis NZ9000 genome by temperature sensitive vector, which makes the NZ-Blue strains become blue on plate with X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside).

References

Simoes-Barbosa, A., Abreu, H., Silva Neto, A., Gruss, A., and Langella, P. (2004). A food-grade delivery system for Lactococcus lactis and evaluation of inducible gene expression. Appl Microbiol Biotechnol 65, 61-67.