Difference between revisions of "Part:BBa K2033002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The BjaI part arises from the soil bacterium Bradyrhizobium japonicum. | + | The BjaI part arises from the soil bacterium Bradyrhizobium japonicum. The designed part by Ryan Muller was cloned into competent DH5AT E. coli cells. These were ligated into the psb1C3 vector and plated, producing the following gel: |
| + | <div style="text-align: center;">[[File:T--Arizona State--Gel4.jpg]]</div> | ||
===Source=== | ===Source=== | ||
Revision as of 22:36, 11 October 2016
isovaleryl-HSL, 3-methyl-N-[(3S)-2-oxooxolan-3-yl]butanamide Sender- BjaI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 294
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The BjaI part arises from the soil bacterium Bradyrhizobium japonicum. The designed part by Ryan Muller was cloned into competent DH5AT E. coli cells. These were ligated into the psb1C3 vector and plated, producing the following gel:
Source
Bradyrhizobium japonicum
References
Lindemann, A., G. Pessi, A. L. Schaefer, M. E. Mattmann, Q. H. Christensen, A. Kessler, H. Hennecke, H. E. Blackwell, E. P. Greenberg, and C. S. Harwood. "Isovaleryl-homoserine Lactone, an Unusual Branched-chain Quorum-sensing Signal from the Soybean Symbiont Bradyrhizobium Japonicum." Proceedings of the National Academy of Sciences 108.40 (2011): 16765-6770. Web.