Difference between revisions of "Part:BBa K1949050:Design"

 
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===Design Notes===
 
===Design Notes===
−
sequence confirmed
+
A point mutation is introduced to <i>amiE</i> to remove the <i>Eco</i>RI recognition site; the 291th nucleotide  was changed from A to a G.
  
  
  
 
===Source===
 
===Source===
 +
The DNA fragment of <i>amiE</i> was artificially synthesized.
  
−
This gene was isolated from Acinetobacter sp. strain Ooi24.
 
−
fwd:
 
−
rev:
 
  
 
===References===
 
===References===

Revision as of 19:09, 11 October 2016


amiE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A point mutation is introduced to amiE to remove the EcoRI recognition site; the 291th nucleotide was changed from A to a G.


Source

The DNA fragment of amiE was artificially synthesized.


References