Difference between revisions of "Part:BBa K1968002:Design"

 
 
Line 1: Line 1:
  
 +
__NOTOC__
 +
<partinfo>BBa_K1968002 short</partinfo>
 +
 +
<partinfo>BBa_K1968002 SequenceAndFeatures</partinfo>
 +
 +
 +
===Design Notes===
 +
5 random nucleotides (TACTA) were added to the 3’ end of the RBS* sequence in order to allow for easier binding to the ribosome. This part contains two MoClo fusion sites, B at the 5' end and C at the 3' end.
 +
A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
 +
 +
 +
 +
===Source===
 +
 +
The sequence of this part was outlined in (1). This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing.
 +
 +
===References===
 +
(1) Heidorn, T., Camsund, D., Huang, H. H., Lindberg, P., Oliveira, P., Stensjo, K., & Lindblad, P. (2011). Synthetic biology in cyanobacteria engineering and analyzing novel functions. Methods Enzymol, 497, 539-579. doi: 10.1016/B978-0-12-385075-1.00024-X

Latest revision as of 18:45, 11 October 2016


Synechocystis RBS* Phytobrick


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

5 random nucleotides (TACTA) were added to the 3’ end of the RBS* sequence in order to allow for easier binding to the ribosome. This part contains two MoClo fusion sites, B at the 5' end and C at the 3' end. A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.


Source

The sequence of this part was outlined in (1). This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing.

References

(1) Heidorn, T., Camsund, D., Huang, H. H., Lindberg, P., Oliveira, P., Stensjo, K., & Lindblad, P. (2011). Synthetic biology in cyanobacteria engineering and analyzing novel functions. Methods Enzymol, 497, 539-579. doi: 10.1016/B978-0-12-385075-1.00024-X