Difference between revisions of "Part:BBa K1968002:Design"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K1968002 short</partinfo> | ||
+ | |||
+ | <partinfo>BBa_K1968002 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | ===Design Notes=== | ||
+ | 5 random nucleotides (TACTA) were added to the 3’ end of the RBS* sequence in order to allow for easier binding to the ribosome. This part contains two MoClo fusion sites, B at the 5' end and C at the 3' end. | ||
+ | A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. | ||
+ | |||
+ | |||
+ | |||
+ | ===Source=== | ||
+ | |||
+ | The sequence of this part was outlined in (1). This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing. | ||
+ | |||
+ | ===References=== | ||
+ | (1) Heidorn, T., Camsund, D., Huang, H. H., Lindberg, P., Oliveira, P., Stensjo, K., & Lindblad, P. (2011). Synthetic biology in cyanobacteria engineering and analyzing novel functions. Methods Enzymol, 497, 539-579. doi: 10.1016/B978-0-12-385075-1.00024-X |
Latest revision as of 18:45, 11 October 2016
Synechocystis RBS* Phytobrick
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
5 random nucleotides (TACTA) were added to the 3’ end of the RBS* sequence in order to allow for easier binding to the ribosome. This part contains two MoClo fusion sites, B at the 5' end and C at the 3' end. A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Source
The sequence of this part was outlined in (1). This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing.
References
(1) Heidorn, T., Camsund, D., Huang, H. H., Lindberg, P., Oliveira, P., Stensjo, K., & Lindblad, P. (2011). Synthetic biology in cyanobacteria engineering and analyzing novel functions. Methods Enzymol, 497, 539-579. doi: 10.1016/B978-0-12-385075-1.00024-X