Difference between revisions of "Part:BBa I760005"
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Was used by Saint-Petersburg Team for constructing a copper biosensor system. | Was used by Saint-Petersburg Team for constructing a copper biosensor system. | ||
| − | This part was updated by Oxford iGEM in 2016 by adding on an long upstream sequence. The resulting part was functional. | + | This part was updated by Oxford iGEM in 2016 by adding on an long upstream sequence. The resulting part was functional. <a href="https://parts.igem.org/Part:BBa_K1980000">here.</a> |
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Revision as of 17:26, 11 October 2016
Cu-sensitive promoter
Promoter from the copper-sensitive CusR/CusS two component signal system. (E.Coli)
Usage and Biology
This nucleotide sequence is believed to be able to bind with phosphorylated CusR transcription factor in E.coli. CusR protein is phosphorylated by CusS transmembrane protein in a case of high extracellular concentration of copper ions. After phosphorylation CusR interacts with described DNA sequence and activates the transcription of CusA, CusB, CusC and CusF genes coding the proteins of copper metabolic system. Was used by Saint-Petersburg Team for constructing a copper biosensor system.
This part was updated by Oxford iGEM in 2016 by adding on an long upstream sequence. The resulting part was functional. <a href="https://parts.igem.org/Part:BBa_K1980000">here.</a>
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]