Difference between revisions of "Part:BBa K1897012:Design"

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===Source===
 
===Source===
  
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Listeriolysin O (LLO) is derived from <i>Listeria monocytogenes</i> and is initially obtained from <partinfo>BBa_K177026</partinfo>. However, after sequencing, the results obtained for sequencing of <partinfo>BBa_K177026</partinfo> did not appear to match that given on the page. We went on to synthesize new LLO (for <partinfo> BBa_K1897013</partinfo>) with the results obtained from sequencing.
+
Listeriolysin O (LLO) is derived from <i>Listeria monocytogenes</i> and is initially obtained from <partinfo>BBa_K177026</partinfo>. However, after sequencing, the results obtained for sequencing of <partinfo>BBa_K177026</partinfo> did not appear to match that given on the page. We went on to synthesize new LLO (for <partinfo>BBa_K1897013</partinfo>) with the results obtained from sequencing.
  
 
===References===
 
===References===

Revision as of 15:13, 11 October 2016


Listeriolysin O CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1380
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is codon optimised for expression in Escherichia coli.


Source

Listeriolysin O (LLO) is derived from Listeria monocytogenes and is initially obtained from BBa_K177026. However, after sequencing, the results obtained for sequencing of BBa_K177026 did not appear to match that given on the page. We went on to synthesize new LLO (for BBa_K1897013) with the results obtained from sequencing.

References