Difference between revisions of "Part:BBa K1927000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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We added prefix and suffix to the gene so that we could perform restriction digestion and Gibson Assembly. We had to design the gene so that it will get the correct readingframe.  
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We designed this biobrick without any promotor so the part would be less dangerous to deal with.
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===Source===
 
===Source===

Revision as of 10:55, 11 October 2016


Beta-lacatamase enzyme called blaNDM-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 716


Design Notes

We designed this biobrick without any promotor so the part would be less dangerous to deal with.

Source

The sequence is collected from pUC19 vector online and sent for synthesis to IDT.

References